Gabor.vpCorelVentura 7.0 - doiSerbia It was assumed that uterine stem cells are responsible for the unique regenerative capacity of uterine. HRMA for genotyping of mutant allele T for SLC35A3 gene in Holstein cattle is an effective. Rotor-Gene. 6000 Series Software version 1.7 Corbett Research, Australia. Coulter, USA according to the manufacturer's manual and sequenced on an. Amplification curves of 177 bp PCR products on the Rotor-Gene 6000.
Biology Free Full-Text Development of a qPCR Method to. Therefore, the aim of the present study was to investate the expression of the pluripotent stem cell markers in the mice uterine tissue during different stages of estrous cycles. QPCR analysis was performed using a Corbett Rotor-Gene 6000. the Corbett Rotor-Gene 6000 Application Software, version 1.7 Qiagen.
Expression of pluripotent stem cell markers in mouse uterine tissue. Twelve virgin female NMRI mice (6 to 8 weeks old) were considered at proestrus, estrus, metestrus and diestrus according to the cell types observed in the vaginal smear and underwent hysterectomy operation. Real-time PCR was performed on a Rotor-Gene 6000 machine Corbett Life. Rotor Gene 6000 series software version 1.7; Corbett Life Science, Corbett, USA. As soon as the hormonal levels decrease, the endometrium does not support.
Improving the Efficiency of DNA Casework Analysis through Simple. Quantitative real-time polymerase chain reaction (PCR) and immunohistochemical staining for pluripotent stem cell markers (SOX2, OCT4, KLF4, and NANOG) were performed. Time PCR and HRM melting in a Corbett Rotorgene RG6000. The three. During extensive experiments to optimize this new real-time version of the assay, it became clear. the RG6000 Series software, Version 1.7, Build 61. Bins need to be. fragments to support a full amplification using commercial STR kits. The new.
Aggregatibacter Actinomycetemcomitans reduced GNAS Gene. Immunofluorescence staining revealed that expression and localization of the pluripotency markers SOX2, OCT4, KLF4, and NANOG at the protein level were not different throughout estrous cycle. Following the method provided by Corbett Life Science using. Rotor-Gene 6000 software. Details were described in an operation manual version 1.7.40, 2006.
PDF1552K - Wiley Online Library Also, m RNA of pluripotency markers was detected in all tested samples. Manually selecting it using image analysis software IMAGEJ. Wilmington, MA in a Rotor-Gene 6000 Corbett Life Sci- ence. software version 1.7.75.
Full Text PDF However, there were no snificant differences in their genes expression at each stage and during the estrous cycle. Used to detect the daaD gene in the DAEC strains isolated. Results Of. cording to manufacturer's instructions. The specific. formed on Rotor-Gene 6000 Corbett research, Australia. version 1.7 Fure 1 14, 15. 3.3.3.